Cells, Free Full-Text

High-resolution 3D images of organelles are of paramount importance in cellular biology. Although light microscopy and transmission electron microscopy (TEM) have provided the standard for imaging cellular structures, they cannot provide 3D images. However, recent technological advances such as serial block-face scanning electron microscopy (SBF-SEM) and focused ion beam scanning electron microscopy (FIB-SEM) provide the tools to create 3D images for the ultrastructural analysis of organelles. Here, we describe a standardized protocol using the visualization software, Amira, to quantify organelle morphologies in 3D, thereby providing accurate and reproducible measurements of these cellular substructures. We demonstrate applications of SBF-SEM and Amira to quantify mitochondria and endoplasmic reticulum (ER) structures.

An integrated in vivo/in vitro framework to enhance cell-free biosynthesis with metabolically rewired yeast extracts

Polimer Program Get File - Colaboratory

Amyloid β directly interacts with NLRP3 to initiate inflammasome activation: identification of an intrinsic NLRP3 ligand in a cell-free system, Inflammation and Regeneration

Cancers, Free Full-Text

Advances and applications of cell-free systems for metabolic production - ScienceDirect

Textile-embedded cell-free biosensors

Cell-free synthetic biology: Engineering in an open world - ScienceDirect

An illustration of the full-duplex cell-free massive MIMO system.

Circulating Tumor Cells, Disease Progression, and Survival in Metastatic Breast Cancer