Expediting Antibody Discovery with a Cell and Bead Multiplexed

A high-throughput, multiplexed cell and bead-based competition assay that identifies and quantitates mouse IgG isotypes and assesses cell health is developed that can reduce the time to antibody cloning and production. With advances in molecular engineering and humanization, monoclonal antibodies are one of the fastest-growing classes of biopharmaceuticals. During antibody discovery, antibody from hybridoma or primary B-cell supernatants is screened for the desired binding characteristics, and secondary screens measure antibody function and concentration, identify immunoglobulin G (IgG) isotype, and assess cell health. In order to expedite the antibody discovery process, we developed a high-throughput, multiplexed cell and bead-based competition assay that identifies and quantitates mouse IgG isotypes and assesses cell health. No differences in assay performance were observed between single and multiplex formats. The linear range of the assay was from 0.5 to 50 µg/mL, and washing was not required, decreasing assay time and variability. Slight modifications to the protocol allowed quantification of dilute antibody supernatants (0.1–5 µg/mL). Using hybridoma cultures, we showed that cell viability measurements in the assay did not interfere with the bead-based IgG measurements. The assay described here is a simple mix-and-read, no-dilution screen that can reduce the time to antibody cloning and production. The high-content data can differentiate monoclonal and polyclonal wells, determine IgG quantity for downstream functional assays, provide isotype information, and monitor cell proliferation and viability.

Single-cell sequencing of plasma cells from COVID-19 patients

CiteAb introductory guides: Multiplexed antibody based imaging

The Role of Single-Cell Profiling and Deep Immunophenotyping in

ELISA in the multiplex era: Potentials and pitfalls - Tighe - 2015

A Scalable Pipeline for High-Throughput Flow Cytometry - SLAS

Single B Cell Antibody Technologies

Multiplexed protein measurement: technologies and applications of

A Magnetically Driven Tandem Chip Enables Rapid Isolation and

Comparison of purified antibodies with antibodies in cell culture

Uploads from Cell Signaling Technology, Inc.

Luminex Discovery Assays R&D Systems, a Bio-Techne Brand

An immunogenic cell injury module for the single-cell multiplexed

Single-cell sequencing of plasma cells from COVID-19 patients

Single B cell technologies for monoclonal antibody discovery

iQue® Mouse IgG Type and Titer Assay - Intellicyt